Fans of the six clubs involved protested widely against the Super League
The Premier League is set to introduce a new Ownership Charter to stop future attempts to join a breakaway Super League.
All owners must adhere to the league’s new “Commitment to the Basic Principles” rule, with violations being punished with “substantial” sanctions.
The football association said an investigation into the six clubs has begun.
“The actions of some clubs must not lead to such a division and disruption,” said the Premier League.
“We are determined to find out the truth about what happened and hold these clubs accountable for their decisions and actions.”
The football association added: “We have written to all clubs to officially request all relevant information and evidence regarding their participation.
“As soon as we have the necessary information, we will consider the appropriate steps to take.”
The Super League proposal, which included some of Europe’s biggest clubs, collapsed within 72 hours of widespread criticism from fans, players, governing bodies and politicians.
Various sanctions were called for against the clubs, including point deductions, relegation and bans on European competitions.
The executives of the six clubs that were involved in the collapsed runaway were forced to resign from advisory roles in the Premier League.
Most of the clubs apologized and Manchester United Vice Chairman Ed Woodward apologized resigned.
In its statement, the Premier League said it would also “introduce additional rules and regulations to ensure that the principles of the Premier League and open competition are protected”.
Both the FA and the UK top flight said they are asking the government for help in putting in place laws to protect the football pyramid and “the integrity of the football community”.
“The events of the past two weeks have challenged the foundations and determination of English football,” the Premier League said.
“These measures are designed to stop the risk of breakaway leagues in the future.”
The Premier League also said it “recognizes the strength of feeling” among fans but called on protests to remain peaceful.
It follows the Protest by Manchester United fans on Sunday This resulted in two police officers injured and the club’s game against Liverpool being postponed.